specimen collection, preservation and transportation ppt

LABORATORY SPECIMEN COLLECTION & HANDLING. Frozen specimens thawing during transport b. Unspun specimens transported >2 hours from collection Specimen Collection Patient results are only as good as the specimen collected. Avoid a probing, traumatic venipuncture Use a larger bore needle (20 gauge) Draw from antecubitalfossa rather than the hand , 2023 SlideServe | Powered By DigitalOfficePro, LABORATORY SPECIMEN COLLECTION & HANDLING, - - - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - - -. If there is likely to be a delay to more than a few hours in the specimens reaching the laboratory, suspend about one gram of feces in 9 ml of phosphate-buffered saline. Disposable Virus Sampling Tube Kit MVTM-5A (Inactivate) is used for the collection, transportation and storage of samples.It is composed of the tube with 3ml solution,and the swab +86-18926519827 | info@medicoswab.com 0 A specimen of nasopharyngeal secretion can also be obtained by using a sterile mucus extractor. Ideally microbiological specimens should be collected before beginning any treatment such as antibiotics or using antiseptics. Mysore University. Do you have PowerPoint slides to share? PAP smear requests must contain, in addition to the above listed information, the following: Date of last menstrual period (LMP) Previous abnormal reports Treatment and/or biopsy information. W\.\f0q[T`5Z)/L;:&2#d+38::,:::2: A"\45V7X9H500304,;1!hiSUf%98S p ;xD d;7 Assess the need for sample recollection and/or rejection. ( See Blood Specimens: Chemistry and Hematology - Consideration for Single and Multiple Sample Collection.) Collecting the urine specimen is awkward but not in itself uncomfortable (An infection, however, can create a burning sensation during urination.). 7. specimen emails. All specimen containers must be transported in a double-sided, self-sealing polythene bag with one compartment containing the laboratory request form and the other the specimen. hbbd```b``"j@$SX&/O`r?VDtE$ydK000R k3 May perform venipunctures, including blood culture collection, and fingersticks ensuring proper /labeling of specimens and using vein preservation techniques. At higher temperatures samples should be kept in a refrigerator (4-8oC) until they can be shipped to the laboratory on ice. satisfying kochs second postulate. 5-3: Collection and preservation The collection of appropriate and optimum samples is the responsibility of the laboratory, even though the actual collection process is often carried out by persons . If possible centrifuge at 2000 g for 15 minutes, and then transfer the supernatant fluid to a clean leak-proof container. Patients Preparation factors ,cont. A request form should include the following information: Sooner the specimens reach the laboratory the better. 1-2 gm quantity is sufficient. Source of specimen. - The requisition form had the patient's name, and ordering physician. Long term storage: -70C/-190C(liquid nitrogen), Preserves viral infectivity within the specimen, Mismatch of information on the label and the request. f47ztf9!oKBt-~Um93V3)7u;ZF-#H1M Store at 2-8 o C. Many drugs will have "peak" and "trough" levels that vary according to dosage levels and intervals. If unable to collect 3 mL of urine, collect in sterile specimen container (37777) or yellow top tube (104184) and transport urine specimens to the Microbiology Laboratory or refrigerate within 30 minutes. Either, Blood or chocolate agar supplemented with, B. pertussis will not grow on media containing, Dont learn this stuff for me at this point. surgical procedures), will need a . Improper collection and handling of samples lead to sample contamination or degradation, finally resulting in inaccurate analysis. Urine Specimen Collection and Handling 1. Specimen Collection & Transport Chapter 45,46,47 Basic Microbiology for Nursing Teaching group Specimen qualityTransport Lab Analysis Proper Diagnosis Poorly collected /Transported Fail to isolate the causative microorganisms Recover contaminants/normal flora Sources of Errors Pre-analytical stage Analytical stage Post- analytical stage https://cmr.asm.org/content/cmr/3/2/120.full.pdf, https://www.researchgate.net/publication/284875818_Specimen_collection_transport_and_processing_Virology, https://www.laboratoryalliance.com/healthcare-providers/laboratory-services/specimen-collection-documents/virology-specimen-collection-guidelines/, https://www.who.int/ihr/publications/CDS_EPR_ARO_2006_1.pdf, https://www.who.int/influenza/rsv/rsv_collection_transport_storage_samples/en/, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC358147/https://reallaboratory.com/product/virus-transport-and-preservation-medium-inactivated/, https://www.cdc.gov/coronavirus/2019-ncov/lab/guidelines-clinical-specimens.html, https://watermark.silverchair.com/22-5-766.pdf, https://www.thelancet.com/journals/lancet/article/PIIS0140-6736(20)30557-2/fulltext. Collect a specimen by passing a sterile cotton wool swab, attached to a pent piece of thin wire, through the floor of the nasal cavity as far as the nasopharynx. Tubes must be filled to 3 mL to prevent inhibition of bacterial growth. A four-fold rise in titer between paired sera establishes a positive result. Be aware that the microbes, Good microbiology cannot be performed on a bad, Surface cleansing with alcohol and iodine for, Rinsing oral cavity with water before collecting, Touching only inflamed area of the throat, Cleansing the external genitalia before having, Use your head think like a microbe and it will, Klebsiella pneumoniae causes acute bacterial, Collecting saliva instead of sputum can lead to a, Specimens must be from the actual infection site, Sputum (deep cough) versus saliva (superficial), Drainage from deeper sites versus scabs on top, Aspiration of pus after skin decontamination is, Collect the specimen prior to administration of, Acute symptomatic phase is the most productive, S. typhi (typhoid fever) can be cultured from, S. typhi can be cultured from feces and urine in, S. typhi usually cannot be cultured after the, Serology is method of choice for diagnosing, The number of specimen should be appropriate. Nasopharyngeal specimens are required to diagnose respiratory syncytial virus infection, and when necessary to investigate infections caused by influenza and parainfluenza viruses, measles, rubella virus, adenoviruses, and enteroviruses. sample collection. It's FREE! Errors Anticoagulant cross contamination Citrate : If drawing a blue top tube for coagulation tests, (other than PT and PTT), a red tube (2-3 cc) must be drawn first to avoid contamination from tissue thromboplastin, which can yield false coagulation Results. With only a rare exception, virus cultures are not worthwhile for specimens collected more than 7 days after the onset of illness. The type of specimen, the appropriate time to obtain the sample, the way of sampling, the storage and transport are critical points in the diagnostic process. Will Drinking Water Lower Fasting Blood Sugar? <> What is the collection order of tubes? Midstream "Clean-Catch" Urine Specimen 4.3. After few seconds, slowly withdraw the swab and immerse the swab in a container of sterile VTM, cutting off swab wire to allow the bottle cap to be replaced tightly. COLLECTION AND TRANSPORT By accepting, you agree to the updated privacy policy. Postural effects are accentuated in patients with edema and low plasma protein values. More than one routine throat, sputum, feces, However, a single blood culture is not adequate, The number of specimens should be appropriate, A single blood culture is never adequate. The specimen volume and the suspected organism's nature influence the acceptable transport time. Serene Pearl Follow Advertisement Recommended Sample collection and safety procedure in laboratory Priyanka Buragohain 15.9k views 54 slides Place about 4-8 g of feces (small spoon) in a clean, dry, leak-proof container. Requisition form : It should contain the proper information in order to process the specimen. The material consists of five more or less complete bodies and five heads that were in varying states of preservation and completeness. Proper patient identification MANDATORY. Looks like youve clipped this slide to already. If so, just upload it to PowerShow.com. Besides, collection, handling and preservation of samples are also very important and critical. 2022. 4. the lab or pathology department does countless types, Laboratory Diagnosis of Infection - . Select a suitable site for venipuncture Prepare the equipment, the patient and the puncture site. Smears of expectorated sputum should be made. Viral Specimens or samples collection plays a vital role in laboratory diagnosis. The recovery of Streptococcus pneumoniae, If it is a hospitalized patient, Pseudomonas, Non-bacterial organisms such as opportunistic, Viruses are not an infrequent cause of LRTs, Mycoplasma pneumoniae is the cause of primary, Legionellosis (caused by Legionella pneumophila), Mycobacteria, especially M. tuberculosis in the, Sheeps blood agar (SBA) is used for routine, S. pneumoniae, which is by far the most common, Chocolate agar is included because H. influenzae, MacConkey agar is included to isolate enteric, After assessing the quality of the sputum the, If there appears to be is a predominating, An experienced microbiologist often can make, Septicemia bacteria actively growing in the, Bacteremia bacteria being transported via the, Morbidity mortality occur in both cases, but, Occult bacteremia bacteremia with persistent, Intermittent bacteremia occurs when microbes are, Persistent bacteremia patient is constantly, Toxemia septicemia with persistent systemic, Blood sample contamination with skin microbes is, Apply 12 iodine antiseptic, allow to dry 1-2, Taking multiple blood samples increases the, Ideally a blood culture should be taken just as, If this is not possible (not all patients with, If antibiotics need to be given immediately, two, The volume of blood collected is the most, The minimum volume of blood for a single adult, 20 to 30 mL per sample is advocated by many, For each 1mL increase of blood/culture, there, Blood contains many natural antimicrobial, Additionally, when the blood is collected, In order to negate these antimicrobials blood, Common blood culture media are liquid and contain, The ideal blood to media ratio (see above) is 1, Many commercially automated blood culture systems, Use of anticoagulants is essential to prevent, Anticoagulants used must not inhibit the growth, In addition to anticoagulant properties, SPS, SPS interferes with the antibacterial action of, SPS may inhibit the growth of certain pathogenic, A blood sample is distributed between two (or, One bottle of media, best suited for growing, The other bottle is not vented (?CO2 ?O2) and, Macroscopic examination bottles are observed for, An aliquot of a well-mixed broth and blood is, Blind subculture of aerobic bottles showing no, Chocolate agar is the best medium for routine, Chocolate agar subcultures are incubated under, Advantages of a conventional broth-based-non-autom, The whole process is labor intensive because, Results of positive conventional blood cultures, Blood samples are processed as usual, inoculated, Once placed in the incubator/monitor/computer, Monitoring is continuous (usually once every ten, Time course data - growth curve - kinetics, Visual and/or audible results signals are given, Routine blind subcultures are not necessary, Incubation times are usually shorter than for, Optical density meter to detect turbidity, CO2 evolution (or consumption?) 3. Oxalate : Interferes with cell membranes, Reasons for a Rejected Specimen No Test Requisition Form (TRF) Test not written on TRF Submitted wrong specimen for test requested Quantity Not Sufficient (QNS) Missing or inadequate identiification Clotted, Avoiding Common Errors in Specimen Collection Insufficient quantity Hemolysis Failure to use the correct container for specimen collection Inaccurate and incomplete patient guidance Failure to label a specimen correctly and to provide all pertinent information Failure to tighten specimen container lids, resulting in leakage and/or contamination of specimen, To prevent a hematoma: Puncture only the uppermost wall of the vein Make sure the needle fully penetrates the upper most wall of the vein. Stress: May cause transient elevation in white blood cells (WBC's) and elevated adrenal hormone values (cortisol and catecholamines). dr ahmad mohammad ashshi (aibms) consultant virologist head of, T-SPOT PROTOCOL - . BD offers Vacutainer blood sample collection tubes in both glass and plastic. collecting liquid specimens in a needle and syringe 10 General guidelines Prompt processing is important The quicker the specimen is processed the better Pathogens may die in transit thus leading to false negative results (ideally specimens for culture should arrive in the lab within 30 minutes of collection) prior to antibiotics or dosing schedules). Refrigerated immediately at 4C. n&)AE(y CtQuIx`_9BP&%o-)}pJ6 sd.%E>KPL\,P)4v09F.r#m".z?/G h:"dgmzQeO[zq2y]Q#Fp6:l2o;)FWPY(b;9OI+={pG~x2% nH6Ixj?bt*Gx||$w0eRT@(YlvHGdUiB0 Knw9+ h It is critical that adequate Proper preservation ensures a high quality specimen, which increases the quality of information the specimen contains, and increases the value of the maintenance of the specimen. Details about immunization and antimicrobial therapy. Free access to premium services like Tuneln, Mubi and more. Specimen Collection Procedures: Cytology Cytology Collection Manual CYTOLOGY LABORATORY University of Florida Health Pathology Laboratories Hours of operation: Monday - Friday | 8 a.m. - 4:30 p.m. The SlideShare family just got bigger. Collect skin scrapings in a dry sterile container. If so, share your PPT presentation slides online with PowerShow.com. PowerPoint presentation 'Specimen Collection and Transport' is the property of its rightful owner. A nurse must do the relabeling. Review procedure manual fill in requisitions. Specimen labelling and processing: microbiology. Uses of laboratory test To provide information relating to a clinical diagnosis To investigate the etiology of a clinical condition To study complications of a disease or its treatment To provide prognostic information To monitor natural history or response to Rx. Now customize the name of a clipboard to store your clips. Specimen collection and transport Dr.Ashna Ajimsha Ashna Ajimsha 371 views Sample Collection In Microbiology Anuj Sharma 44.3k views Sample collection and safety procedure in laboratory Priyanka Buragohain 16k views Specimen collection and preservation Serene Pearl 48.1k views Similar to Collection and transport (20) Conversely, moving from upright to supine can have a dilutional effect owing to an increase in plasma volume. The SlideShare family just got bigger. Specimens such as urine and sputum are generally unsuitable for viruses isolation. of specimen at the right time, Place the specimen in the appropriate place for, Document/record specimen sent and anything, Delegated to those instructed in skill if, Ordered ac, pc, hs, fasting, before insulin, Antiseptic swab ( allow to dry completely), 3 benefits to measuring progress and results, Allows us to make improvements along the way, Helps us focus on what we value and where we are, Gives info what is happening along the way and. Two serum specimens (paired sera) are necessary to detect a rise in the titer of the Ab. Timed urine specimens. Can falsely elevate potassium results if drawn before a greentop tube. Conducting regular self-management tasks such as medication and insulin intake, blood sugar checkup, diet observance, and physical exercise are really demanding. The site provides information on human evolution evidence, research, and human characteristics, along with resources for education. Perform the venipuncture. All blood, whether unlabelled or mislabeled, intended for cross matching, must be redrawn, without exception. Previously parataxonomy of these titanosaur clutches . If the specimen is irreplaceable the ordering physician must attest to the correct patients identity and must authorize (on the specimen relabeling form) affixation of the correct label by an attendance nurse. Collect the sample in the appropriate container. Remember to wash hands well after collecting the specimen. Whatever your area of interest, here youll be able to find and view presentations youll love and possibly download. 379 0 obj <>/Filter/FlateDecode/ID[<0418542909B6434FA5DC5F39696DAE8B>]/Index[363 31]/Info 362 0 R/Length 89/Prev 695974/Root 364 0 R/Size 394/Type/XRef/W[1 3 1]>>stream of containers if more than one, pertinent previous cytological and/or histological examination are declared in request. Transport to the microbiology laboratory. NE P S OR SP CIM N This study provides information about the sales and revenue during the historic and forecasted period of 2015 to 2026. Mix specimen thoroughly, then insert a sterile, Place the filled loop flat onto the agar and drag, Streak perpendicular to the 1 streak in a way, Plating 0.01mL of urine is equivalent to making a, For statistical validity, the number of colonies, The reporting unit is CFU/mL and not colonies, Making serial dilutions of urine in sterile water, Even though this method is very accurate it is, The kind of accuracy this method allows is not, Properly collected catheterized urine rarely is, However, the process of catheterizing is a risk, If the patient is going to be catheterized for, Urine taken from an indwelling catheter must not, The specimen should be taken aseptically with a, Again, this is not recommended for routine, It is now known that clean-catch counts are, The urethral opening is colonized with a variety, Collection of mid-stream urine prevents much of, Clean-catch applies to urine that does not make, This is easy for males, but often requires, These samples are best taken first thing in the, Urine specimens must be processed within 2 hrs of, Suprapubic aspiration (SPA), another method of, Suprapubic aspirated urine is the only type of, Suprapubic aspiration is done by qualified, Then a needle is inserted through the skin and a, Cystoscopy is a procedure that uses a fiber-optic, During this exam urine specimens can be collected, Properly collected, freshly voided urine from a, A given UTI is usually caused by a single species, If gt 3 organisms grow in a urine specimen (other, If 2 different organisms grow at a colony count, Direct microscopic examination of urine for, Occasionally a physician will request a Gram, A drop of unspun urine is placed on a microscope, If the number of microbial cells averages one or, Meningitis is inflammation of the meninges. Some sex hormones need special timing as regard cycle : (1)FSH,LH (3rd day of cycal {follicular phase}) (2)Progesterone (21 day of cycle{ luteal phase}) Other Factors: Age, gender, and pregnancy have an influence on laboratory testing. A 3D digital collection of fossils and artifacts is also available online. laboratory diagnosis of, Diagnostic Sampling - . Maio. By dr. mervat, Establishing diagnostic microbiology laboratory - . Normally thin, nonpurulent, whitish or clear, Tests to determine abnormal cells or infection, Culture determines pathogenic microorganisms, Sensitivity determines the antibiotics to which, Swab must advance into nasopharynx to ensure, Ordered to identify organisms growing in sputum, Induration palpable, elevated, hardened area, The elderly freq. There are some medications and other substances that can alter the results of the analyses. WHAT IS THE MOST IMPORTANT THING YOU NEED FOR SPECIMEN COLLECTION??? role of the nurse. Click here to review the details.
Disadvantages Of Monogamy Family, Where Was The Video Who's Gonna Fill Their Shoes Filmed, Dr 4746 Colorado Department Of Revenue, Writing Equations Of Lines Activity, Articles S